GB/T 17819-2017 Translated English of Chinese Standard. (GBT 17819-2017, GB/T17819-2017, GBT17819-2017): Determination of Vitamin B12 in Additive Premix - High Performance Liquid Chromatographyhttps://www.chinesestandard.net, 19 µ.¤. 2018 - 10 ˹éÒ This Standard specifies the method of high performance liquid chromatography in the determination of vitamin B12 in additive premix. This Standard is applicable to the determination of vitamin B12 in compound premix feed and vitamin premix feed. The detection limit of the method in this Standard is 0.1 mg/kg; the quantitative limit is 0.5 mg/kg. |
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Scope | 4 |
Instruments and Equipment | 6 |
Result Calculation | 8 |
Appendix A Informative Standard Chromatogram of Vitamin B12 | 10 |
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100 mL brown 50 mL centrifuge accordance with GB/T accurate to 0.001 Accurately add 20 Acetic acid Acetonitrile additive premix feed Adopt external standard Amino column arithmetic mean value B12 in Additive brown volumetric bottle calculated in accordance chromatographic purity column Chromatographic column column with equivalent compound premix feed concentration of vitamin content of B12 Determination of Vitamin determination results obtained equivalent performance feed whose vitamin fluid of vitamin g accurate hexane sulfonate solution high performance liquid independent determination results mg/kg mL brown volumetric mL of water Mobile phase Nitrogen obtained under repeatability performance liquid chromatography phase extraction column phosphate purification r/min reach dryness sample solution shake it twice Sodium hexane sulfonate solid phase extraction specified date standard stock solution standard working fluid take the supernatant ultrasonic water bath vitamin B12 content vitamin B12 standard vitamin premix water in GB/T water to dilute wavelength of 361 Weigh-take 2 g