Proteomic Profiling and Analytical Chemistry: The CrossroadsPawel Ciborowski, Jerzy Silberring Elsevier, 2 มี.ค. 2016 - 298 หน้า Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
|
เนื้อหา
1 | |
7 | |
25 | |
4 PROTEIN EXTRACTION AND PRECIPITATION | 51 |
5 ONLINE AND OFFLINE SAMPLE FRACTIONATION | 63 |
6 IMMUNOAFFINITY DEPLETION OF HIGHLY ABUNDANT PROTEINS FOR PROTEOMIC SAMPLE PREPARATION | 101 |
7 GEL ELECTROPHORESIS | 115 |
MASS SPECTROMETRY | 145 |
10 TOPDOWN PROTEOMICS | 175 |
11 PROTEOMIC DATABASE SEARCH AND ANALYTICAL QUANTIFICATION FOR MASS SPECTROMETRY | 193 |
12 DESIGN AND STATISTICAL ANALYSIS OF MASSSPECTROMETRYBASED QUANTITATIVE PROTEOMICS DATA | 211 |
13 PRINCIPLES OF ANALYTICAL VALIDATION | 239 |
14 VALIDATION IN PROTEOMICS AND REGULATORY AFFAIRS | 253 |
273 | |
Back Cover | 283 |
DATA ACQUISITION AND ANALYSIS | 161 |
ฉบับอื่นๆ - ดูทั้งหมด
Proteomic Profiling and Analytical Chemistry: The Crossroads Pawel Ciborowski,Jerzy Silberring ชมบางส่วนของหนังสือ - 2012 |